| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47754-0.1mg | |||
| IHC-Prediluted | NBP2-48087 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47752-0.1mg | |||
| IHC-Prediluted | NBP2-48078 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47753-0.1mg | |||
| IHC-Prediluted | NBP2-48077 | |||
![Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - High magnification confocal image of HeLa cells stained with fibrillarin antibody, dilution 1:100 from concentrated tissue culture media (Green), and costained with chicken vimentin pAb (Red), dilution 1:1,000. Nuclear DNA is revealed with the DAPI stain (Blue). The fibrillarin antibody shows strong staining of nucleoli in the nucleus, while the vimentin antibody reveals cytoplasmic intermediate filaments.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-38F3-Immunocytochemistry-Immunofluorescence-NB300-269-img0008.jpg "Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - High magnification confocal image of HeLa cells stained with fibrillarin antibody, dilution 1:100 from concentrated tissue culture media (Green), and costained with chicken vimentin pAb (Red), dilution 1:1,000. Nuclear DNA is revealed with the DAPI stain (Blue). The fibrillarin antibody shows strong staining of nucleoli in the nucleus, while the vimentin antibody reveals cytoplasmic intermediate filaments.")
![Western Blot: Fibrillarin Antibody (38F3) [NB300-269] - Analysis of lysates of cell fractions probed with Fibrillarin antibody, dilution 1:500 (Green): [1] protein standard, [2] C6 cytosol, [3] C6 nuclear, [4] HEK293 cytosol, [5] HEK293 nuclear, [6] NIH-3T3 cytosol and [7] NIH-3T3 nuclear fractions. The band at 37kDa corresponds to the fibrillarin protein detected exclusively in the nuclear fractions.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-38F3-Western-Blot-NB300-269-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - The nuclei of hth mutants have dispersed rDNA foci and ectopic nucleoli and produce more rRNA. A) Wild type nuclei stained with anti-Fibrillarin (red) and a specific probe that recognizes the rDNA genomic region (green). B) hth mutant nuclei stained as in A. The mutant nuclei exhibit an increased number of nucleoli with different sizes. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0120662), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-38F3-Immunocytochemistry-Immunofluorescence-NB300-269-img0012.jpg)
![Immunohistochemistry-Paraffin: Fibrillarin Antibody (38F3) [NB300-269] - IHC staining of Fibrillarin in human ovarian cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-38F3-Immunohistochemistry-Paraffin-NB300-269-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - Mouse embryonic fibroblast fibrillarin stained with fibrillarin antibody and Alexa-594 secondary antibody. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-38F3-Immunocytochemistry-Immunofluorescence-NB300-269-img0013.jpg)
![Flow Cytometry: Fibrillarin Antibody (38F3) [NB300-269] - Fibrillarin antibody was tested at 1:400 in HEK293 cells using an Alexa Fluor 488 secondary (shown in green) alongside unstained cells (shown in red).](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-38F3-Flow-Cytometry-NB300-269-img0004.jpg)
![Western Blot: Fibrillarin Antibody (38F3) [NB300-269] - Western blot analysis of Fibrillarin expression on yeast protein extracts using NB300-269.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-38F3-Western-Blot-NB300-269-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - Fibrillarin immunofluorescence in fibroblasts. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-38F3-Immunocytochemistry-Immunofluorescence-NB300-269-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - IF Confocal analysis of MCF7 cells using Fibrillarin antibody (NB300-269, 1:5). An Alexa Fluor 488-conjugated Goat to mouse IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-38F3-Immunocytochemistry-Immunofluorescence-NB300-269-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - The nuclei of hth mutants have dispersed rDNA foci & ectopic nucleoli & produce more rRNA.A) Wild type nuclei stained with anti-Fibrillarin (red) & a specific probe that recognizes the rDNA genomic region (green). B) hth mutant nuclei stained as in A. The mutant nuclei exhibit an increased number of nucleoli with different sizes. C) Quantification of nuclear dots marked with both the rDNA probe & anti-Fibrillarin in wild type & mutant nuclei. 45 nuclei were quantified for each genotype. (p-value: 0,00010132, calculated using T-Test). D) qRT-PCR comparing the levels of 18S RNA transcripts in wild type & Dfhth mutant embryos. The experiment was performed three independent times to be significant (p-value: 0,01046, calculated using T-Test). Error bars in the graph show the standard deviation & have being calculated as in Fig. 1E. The experiment was normalized using the bicoid (bcd) mRNA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25794008), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-269_mouse-monoclonal-fibrillarin-antibody-38f3-310202416205116.jpg)
![Immunocytochemistry/ Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - The nuclei of hth mutants have dispersed rDNA foci & ectopic nucleoli & produce more rRNA.A) Wild type nuclei stained with anti-Fibrillarin (red) & a specific probe that recognizes the rDNA genomic region (green). B) hth mutant nuclei stained as in A. The mutant nuclei exhibit an increased number of nucleoli with different sizes. C) Quantification of nuclear dots marked with both the rDNA probe & anti-Fibrillarin in wild type & mutant nuclei. 45 nuclei were quantified for each genotype. (p-value: 0,00010132, calculated using T-Test). D) qRT-PCR comparing the levels of 18S RNA transcripts in wild type & Dfhth mutant embryos. The experiment was performed three independent times to be significant (p-value: 0,01046, calculated using T-Test). Error bars in the graph show the standard deviation & have being calculated as in Fig. 1E. The experiment was normalized using the bicoid (bcd) mRNA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25794008), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-269_mouse-monoclonal-fibrillarin-antibody-38f3-310202416205175.jpg)
![Immunocytochemistry/ Immunofluorescence: Fibrillarin Antibody (38F3) [NB300-269] - The nuclei of hth mutants have dispersed rDNA foci & ectopic nucleoli & produce more rRNA.A) Wild type nuclei stained with anti-Fibrillarin (red) & a specific probe that recognizes the rDNA genomic region (green). B) hth mutant nuclei stained as in A. The mutant nuclei exhibit an increased number of nucleoli with different sizes. C) Quantification of nuclear dots marked with both the rDNA probe & anti-Fibrillarin in wild type & mutant nuclei. 45 nuclei were quantified for each genotype. (p-value: 0,00010132, calculated using T-Test). D) qRT-PCR comparing the levels of 18S RNA transcripts in wild type & Dfhth mutant embryos. The experiment was performed three independent times to be significant (p-value: 0,01046, calculated using T-Test). Error bars in the graph show the standard deviation & have being calculated as in Fig. 1E. The experiment was normalized using the bicoid (bcd) mRNA. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25794008), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-269_mouse-monoclonal-fibrillarin-antibody-38f3-310202416205111.jpg)
![Immunocytochemistry/Immunofluorescence: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nuclear Matrix Protein p84 stained by Nuclear Matrix Protein p84 antibody [5E10] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-5E10-Immunocytochemistry-Immunofluorescence-NB100-174-img0017.jpg "Immunocytochemistry/Immunofluorescence: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nuclear Matrix Protein p84 stained by Nuclear Matrix Protein p84 antibody [5E10] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.")
![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Nuclear Matrix Protein p84 stained by Nuclear Matrix Protein p84 antibody [5E10] diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-5E10-Immunohistochemistry-Paraffin-NB100-174-img0019.jpg)
![Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (30 ug of whole cell lysate) A: HeLa B: HeLa nucleus 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-5E10-Western-Blot-NB100-174-img0008.jpg)
![Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (50 ug of whole cell lysate) A: mouse Cerebellum 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-5E10-Western-Blot-NB100-174-img0010.jpg)
![Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-5E10-Western-Blot-NB100-174-img0011.jpg)
![Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - p84 western in 293T cells. Image from verified customer review.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-5E10-Western-Blot-NB100-174-img0012.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - Lane 1: total lysate, Lane 2: cytoplasmic fraction, Lane 3: membrane fraction, Lane 4: nuclear fraction.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-5E10-Western-Blot-NB100-174-img0013.jpg)
![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - hHpr1-p84-Thoc1 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Nuclear Matrix Protein p84 stained by hHpr1-p84-Thoc1 antibody [5E10] (NB100-174). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-5E10-Immunohistochemistry-Paraffin-NB100-174-img0015.jpg)
![Immunoprecipitation: hHpr1-p84-Thoc1 Antibody (5E10) [NB100-174] - hHpr1-p84-Thoc1 antibody [5E10] immunoprecipitates p84 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of p84 protein expressing HepG2 cells B : Control with 3 ug of pre-immune mouse IgG C : Immunoprecipitation of p84 by 3 ug of p84 antibody [5E10] (NB100-174) 7.5% SDS-PAGE The immunoprecipitated p84 protein was detected by p84 antibody [5E10] (NB100-174). EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-5E10-Immunoprecipitation-NB100-174-img0016.jpg)
![Western Blot: Histone H1 Antibody (HH1/957) [NBP2-45184] - Western Blot Analysis of human heart tissue lysate using Histone H1 Antibody (HH1/957).](http://images.novusbio.com/fullsize/Histone-H1-Antibody-HH1-957-Western-Blot-NBP2-45184-img0010.jpg "Western Blot: Histone H1 Antibody (HH1/957) [NBP2-45184] - Western Blot Analysis of human heart tissue lysate using Histone H1 Antibody (HH1/957).")
![Immunocytochemistry/Immunofluorescence: Histone H1 Antibody (HH1/957) [NBP2-45184] - Confocal Immunofluorescence image of HeLa cells using Green (CF488) and Phalloidin is used to label the membranes.](http://images.novusbio.com/fullsize/Histone-H1-Antibody-HH1-957-Immunocytochemistry-Immunofluorescence-NBP2-45184-img0006.jpg)
![Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Formalin-fixed, paraffin-embedded human Pancreas stained with Histone H1 Antibody (HH1/957).](http://images.novusbio.com/fullsize/Histone-H1-Antibody-HH1-957-Immunohistochemistry-Paraffin-NBP2-45184-img0011.jpg)
![Flow Cytometry: Histone H1 Antibody (HH1/957) [NBP2-45184] - Flow Cytometric Analysis of HeLa cells. Histone H1 Mouse Monoclonal Antibody (HH1/957) followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Histone-H1-Antibody-HH1-957-Flow-Cytometry-NBP2-45184-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H1 Antibody (HH1/957) [NBP2-45184] - Human cell culture cells were fixed with paraformaldehyde, permeabilised and incubated 1 hour at RT with primary antibody. This image was submitted via customer review.](http://images.novusbio.com/fullsize/Histone-H1-Antibody-HH1-957-Immunocytochemistry-Immunofluorescence-NBP2-45184-img0005.jpg)
![Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Human Ovarian Carcinoma stained with Histone H1 Monoclonal Antibody (HH1/957)](http://images.novusbio.com/fullsize/Histone-H1-Antibody-HH1-957-Immunohistochemistry-Paraffin-NBP2-45184-img0001.jpg)
![Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Human Tonsil stained with Histone H1 Monoclonal Antibody (HH1/957)](http://images.novusbio.com/fullsize/Histone-H1-Antibody-HH1-957-Immunohistochemistry-Paraffin-NBP2-45184-img0002.jpg)
![Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Rat Pancreas stained with Histone H1 Monoclonal Antibody (HH1/957)](http://images.novusbio.com/fullsize/Histone-H1-Antibody-HH1-957-Immunohistochemistry-Paraffin-NBP2-45184-img0004.jpg)
![Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Human Tonsil stained with Histone H1 Monoclonal Antibody (HH1/957)](http://images.novusbio.com/fullsize/Histone-H1-Antibody-HH1-957-Immunohistochemistry-Paraffin-NBP2-45184-img0003.jpg)
![Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Histone H1 Antibody (HH1/957).](http://images.novusbio.com/fullsize/Histone-H1-Antibody-HH1-957-Immunohistochemistry-Paraffin-NBP2-45184-img0007.jpg)
![Immunohistochemistry-Paraffin: Histone H1 Antibody (HH1/957) [NBP2-45184] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Histone H1 Antibody (HH1/957).](http://images.novusbio.com/fullsize/nbp2-45184_mouse-monoclonal-histone-h1-antibody-hh1-957-17720241003244.jpg)
![Western Blot: H1F0 Antibody (1415-1) [NBP2-45181] - Western Blot Analysis of human heart tissue lysate using H1F0 antibody (1415-1)](http://images.novusbio.com/fullsize/Histone-H1-Antibody-1415-1-Western-Blot-NBP2-45181-img0009.jpg "Western Blot: H1F0 Antibody (1415-1) [NBP2-45181] - Western Blot Analysis of human heart tissue lysate using H1F0 antibody (1415-1)")
![Immunocytochemistry/Immunofluorescence: H1F0 Antibody (1415-1) [NBP2-45181] - Confocal Immunofluorescence image of HeLa cells using H1F0 antibody (1415-1) stained green (CF488) and Phalloidin is used to label the membranes.](http://images.novusbio.com/fullsize/Histone-H1-Antibody-1415-1-Immunocytochemistry-Immunofluorescence-NBP2-45181-img0008.jpg)
![Immunohistochemistry-Paraffin: H1F0 Antibody (1415-1) [NBP2-45181] - Histone H1 Antibody (1415-1) [NBP2-45181] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with H1F0 antibody (1415-1)](http://images.novusbio.com/fullsize/Histone-H1-Antibody-1415-1-Immunohistochemistry-Paraffin-NBP2-45181-img0007.jpg)
![Flow Cytometry: H1F0 Antibody (1415-1) [NBP2-45181] - Flow Cytometric Analysis of HeLa cells using H1F0 antibody (1415-1) conjugated to goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Histone-H1-Antibody-1415-1-Flow-Cytometry-NBP2-45181-img0003.jpg)
![Immunohistochemistry-Paraffin: H1F0 Antibody (1415-1) [NBP2-45181] - Human Tonsil stained with H1F0 antibody (1415-1)](http://images.novusbio.com/fullsize/Histone-H1-Antibody-1415-1-Immunohistochemistry-Paraffin-NBP2-45181-img0002.jpg)
![Immunohistochemistry-Paraffin: H1F0 Antibody (1415-1) [NBP2-45181] - Human Tonsil stained with H1F0 antibody (1415-1)](http://images.novusbio.com/fullsize/Histone-H1-Antibody-1415-1-Immunohistochemistry-Paraffin-NBP2-45181-img0001.jpg)
![Immunohistochemistry-Paraffin: H1F0 Antibody (1415-1) [NBP2-45181] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with H1F0 antibody (1415-1)](http://images.novusbio.com/fullsize/Histone-H1-Antibody-1415-1-Immunohistochemistry-Paraffin-NBP2-45181-img0004.jpg)
![Immunohistochemistry-Paraffin: H1F0 Antibody (1415-1) [NBP2-45181] - Formalin-fixed, paraffin-embedded human Pancreas stained with H1F0 antibody (1415-1)](http://images.novusbio.com/fullsize/Histone-H1-Antibody-1415-1-Immunohistochemistry-Paraffin-NBP2-45181-img0005.jpg)
![Immunohistochemistry-Paraffin: H1F0 Antibody (1415-1) [NBP2-45181] - Histone H1 Antibody (1415-1) [NBP2-45181] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with H1F0 antibody (1415-1)](http://images.novusbio.com/fullsize/Histone-H1-Antibody-1415-1-Immunohistochemistry-Paraffin-NBP2-45181-img0006.jpg)
![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89670] - Staining in human testis and skeletal muscle tissues using anti-THOC1 antibody. Corresponding THOC1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-Paraffin-NBP1-89670-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89670] - Staining in human testis and skeletal muscle tissues using anti-THOC1 antibody. Corresponding THOC1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89670] - Staining of human cerebral cortex, colon, skeletal muscle and testis using Anti-THOC1 antibody NBP1-89670 (A) shows similar protein distribution across tissues to independent antibody NBP1-89669 (B).](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-Paraffin-NBP1-89670-img0013.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: hHpr1-p84-Thoc1 Antibody [NBP1-89670] - Analysis using Anti-THOC1 antibody NBP1-89670 (A) shows similar pattern to independent antibody NBP1-89669 (B).](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Western-Blot-NBP1-89670-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: hHpr1-p84-Thoc1 Antibody [NBP1-89670] - Staining of human cell line U-251 MG shows localization to nuclear speckles.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89670-img0011.jpg)
![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89670] - Staining of human testis using Anti-THOC1 antibody NBP1-89670.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-Paraffin-NBP1-89670-img0018.jpg)
![Western Blot: hHpr1-p84-Thoc1 Antibody [NBP1-89670] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Western-Blot-NBP1-89670-img0012.jpg)
![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89670] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-Paraffin-NBP1-89670-img0008.jpg)
![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89670] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-Paraffin-NBP1-89670-img0009.jpg)
![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89670] - Staining of human colon.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-Paraffin-NBP1-89670-img0015.jpg)
![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89670] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-Paraffin-NBP1-89670-img0016.jpg)
![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89670] - Staining of human skeletal muscle using Anti-THOC1 antibody NBP1-89670.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-Paraffin-NBP1-89670-img0017.jpg)
![Western Blot: H1F0 Antibody (AE-4) [NBP2-45182] - Histone H1 Antibody (AE-4) [NBP2-45182] - Western Blot Analysis of human heart tissue lysate using H1F0 antibody (AE-4)](http://images.novusbio.com/fullsize/Histone-H1-Antibody-AE-4-Western-Blot-NBP2-45182-img0007.jpg "Western Blot: H1F0 Antibody (AE-4) [NBP2-45182] - Histone H1 Antibody (AE-4) [NBP2-45182] - Western Blot Analysis of human heart tissue lysate using H1F0 antibody (AE-4)")
![Immunocytochemistry/Immunofluorescence: H1F0 Antibody (AE-4) [NBP2-45182] - Immunofluorescence staining of PFA-fixed HeLa cells using H1F0 antibody (AE-4) followed by goat anti-mouse IgG-CF488 (green). Membrane stained by Phalloidin](http://images.novusbio.com/fullsize/Histone-H1-Antibody-AE-4-Immunocytochemistry-Immunofluorescence-NBP2-45182-img0008.jpg)
![Immunohistochemistry-Paraffin: H1F0 Antibody (AE-4) [NBP2-45182] - Histone H1 Antibody (AE-4) [NBP2-45182] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with H1F0 antibody (AE-4)](http://images.novusbio.com/fullsize/Histone-H1-Antibody-AE-4-Immunohistochemistry-Paraffin-NBP2-45182-img0006.jpg)
![Flow Cytometry: H1F0 Antibody (AE-4) [NBP2-45182] - Flow Cytometric Analysis of HeLa cells. H1F0 antibody (AE-4)followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Histone-H1-Antibody-AE-4-Flow-Cytometry-NBP2-45182-img0003.jpg)
![Immunohistochemistry-Paraffin: H1F0 Antibody (AE-4) [NBP2-45182] - Human Tonsil stained with H1F0 antibody (AE-4)](http://images.novusbio.com/fullsize/Histone-H1-Antibody-AE-4-Immunohistochemistry-Paraffin-NBP2-45182-img0002.jpg)
![Immunohistochemistry-Paraffin: H1F0 Antibody (AE-4) [NBP2-45182] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with H1F0 antibody (AE-4)](http://images.novusbio.com/fullsize/Histone-H1-Antibody-AE-4-Immunohistochemistry-Paraffin-NBP2-45182-img0004.jpg)
![Immunohistochemistry-Paraffin: H1F0 Antibody (AE-4) [NBP2-45182] - Formalin-fixed, paraffin-embedded human Pancreas stained with H1F0 antibody (AE-4)](http://images.novusbio.com/fullsize/Histone-H1-Antibody-AE-4-Immunohistochemistry-Paraffin-NBP2-45182-img0005.jpg)
![Western Blot: hHpr1-p84-Thoc1 Antibody [NBP2-16822] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with hHpr-p84-Thoc1 Antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Western-Blot-NBP2-16822-img0011.jpg "Western Blot: hHpr1-p84-Thoc1 Antibody [NBP2-16822] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with hHpr-p84-Thoc1 Antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: hHpr1-p84-Thoc1 Antibody [NBP2-16822] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nuclear Matrix Protein p84 protein stained by Nuclear Matrix Protein p84 antibody [C1C3] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16822-img0010.jpg)
![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP2-16822] - Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-Paraffin-NBP2-16822-img0002.jpg)
![Chromatin Immunoprecipitation: hHpr1-p84-Thoc1 Antibody [NBP2-16822] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-Nuclear Matrix Protein p84 antibody.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Chromatin-Immunoprecipitation-NBP2-16822-img0007.jpg)
![Western Blot: hHpr1-p84-Thoc1 Antibody [NBP2-16822] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Nuclear Matrix Protein p84 antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Western-Blot-NBP2-16822-img0004.jpg)
![Western Blot: hHpr1-p84-Thoc1 Antibody [NBP2-16822] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Nuclear Matrix Protein p84 antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Western-Blot-NBP2-16822-img0009.jpg)
![Immunoprecipitation: hHpr1-p84-Thoc1 Antibody [NBP2-16822] - HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of p84 protein expressing HepG2 cells B : Control with 3 ug of pre-immune rabbit IgG C : Immunoprecipitation of p84 by 3 ug of Nuclear Matrix Protein p84 antibody [C1C3] 7.5% SDS-PAGE The immunoprecipitated p84 protein was detected by Nuclear Matrix Protein p84 antibody [C1C3] diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunoprecipitation-NBP2-16822-img0006.jpg)
![Western Blot: hHpr1-p84-Thoc1 Antibody [NBP1-89669] - Analysis using Anti-THOC1 antibody NBP1-89669 (A) shows similar pattern to independent antibody NBP1-89670 (B).](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Western-Blot-NBP1-89669-img0012.jpg "<b>Independent Antibodies Validation. </b>Western Blot: hHpr1-p84-Thoc1 Antibody [NBP1-89669] - Analysis using Anti-THOC1 antibody NBP1-89669 (A) shows similar pattern to independent antibody NBP1-89670 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: hHpr1-p84-Thoc1 Antibody [NBP1-89669] - Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89669-img0007.jpg)
![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89669] - Staining of human skeletal muscle using Anti-THOC1 antibody NBP1-89669.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-Paraffin-NBP1-89669-img0016.jpg)
![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89669] - Staining of human cerebral cortex shows strong nuclear positivity in neuronal and glial cells.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-Paraffin-NBP1-89669-img0002.jpg)
![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89669] - Staining of human appendix shows high expression.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-Paraffin-NBP1-89669-img0008.jpg)
![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89669] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-Paraffin-NBP1-89669-img0009.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89669] - Staining in human appendix and skeletal muscle tissues using anti-THOC1 antibody. Corresponding THOC1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-Paraffin-NBP1-89669-img0010.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89669] - Staining of human cerebral cortex, colon, skeletal muscle and testis using Anti-THOC1 antibody NBP1-89669 (A) shows similar protein distribution across tissues to independent antibody NBP1-89670 (B).](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-Paraffin-NBP1-89669-img0011.jpg)
![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89669] - Staining of human testis.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-Paraffin-NBP1-89669-img0013.jpg)
![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89669] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-Paraffin-NBP1-89669-img0014.jpg)
![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP1-89669] - Staining of human colon.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-Paraffin-NBP1-89669-img0015.jpg)
![Western Blot: hHpr1-p84-Thoc1 Antibody [NBP3-38514] - Western blot analysis of various lysates using hHpr1-p84-Thoc1(THOC1) Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38514_rabbit-hhpr1-p84-thoc1-pab-101220241224183.jpg "Western Blot: hHpr1-p84-Thoc1 Antibody [NBP3-38514] - Western blot analysis of various lysates using hHpr1-p84-Thoc1(THOC1) Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.")
![Immunocytochemistry/ Immunofluorescence: hHpr1-p84-Thoc1 Antibody [NBP3-38514] - Immunofluorescence analysis of U2OS cells using hHpr1-p84-Thoc1(THOC1) Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38514_rabbit-hhpr1-p84-thoc1-pab-1012202411552836.jpg)
![Immunohistochemistry: hHpr1-p84-Thoc1 Antibody [NBP3-38514] - Immunohistochemistry analysis of paraffin-embedded Mouse lung using hHpr1-p84-Thoc1(THOC1) Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38514_rabbit-hhpr1-p84-thoc1-pab-1012202411592870.jpg)
![Immunohistochemistry: hHpr1-p84-Thoc1 Antibody [NBP3-38514] - Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using hHpr1-p84-Thoc1(THOC1) Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38514_rabbit-hhpr1-p84-thoc1-pab-10122024120613.jpg)
![Immunohistochemistry: hHpr1-p84-Thoc1 Antibody [NBP3-38514] - Immunohistochemistry analysis of paraffin-embedded Rat ovary using hHpr1-p84-Thoc1(THOC1) Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38514_rabbit-hhpr1-p84-thoc1-pab-101220241224156.jpg)
![Western Blot: hHpr1-p84-Thoc1 Antibody [NBP3-38514] - Western blot analysis of various lysates using hHpr1-p84-Thoc1(THOC1) Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-38514_rabbit-hhpr1-p84-thoc1-pab-1012202411545561.jpg)
![Western Blot: Fibrillarin Antibody [NBP2-46881] - Western blot analysis of different cell lysates using chicken pAb to fibrillarin NBP2-46881, dilution 1:5,000, in green: [1] protein standard (red), [2] NIH-3T3, [3] HEK293, [4] HeLa, [5] SH-SY5Y, and [6] C6 cells. The single strong band at ~35kDa correspond to the fibrillarin protein.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Western-Blot-NBP2-46881-img0002.jpg "Western Blot: Fibrillarin Antibody [NBP2-46881] - Western blot analysis of different cell lysates using chicken pAb to fibrillarin NBP2-46881, dilution 1:5,000, in green: [1] protein standard (red), [2] NIH-3T3, [3] HEK293, [4] HeLa, [5] SH-SY5Y, and [6] C6 cells. The single strong band at ~35kDa correspond to the fibrillarin protein.")
![Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody [NBP2-46881] - Confocal immunofluorescent analysis of HeLa cells stained with chicken pAb to fibrillarin, NBP2-46881, dilution 1:10,000 in green and costained with mouse mAb to vimentin, 1:1,000, in red. The blue signal is DAPI staining of nuclear DNA. The fibrillarin antibody stains nucleoli while the vimentin antibody binds to cytoplasmic intermediate filaments.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Immunofluorescence-NBP2-46881-img0001.jpg)
![Immunohistochemistry: Histone H1 Antibody (21NC85) [NB100-65220] - FFPE pig heart stained with NB100-65220 at 1:500.](http://images.novusbio.com/fullsize/Histone-H1-Antibody-21NC85-Immunohistochemistry-NB100-65220-img0002.jpg "Immunohistochemistry: Histone H1 Antibody (21NC85) [NB100-65220] - FFPE pig heart stained with NB100-65220 at 1:500.")
![Western Blot: Fibrillarin Antibody [NBP3-05533] - Western blot analysis of different tissue and cell line lysates using Fibrillarin Antibody at a dilution of 1:2,000, in green: [1] protein standard (red), [2] rat whole brain, [3] mouse whole brain, [4] NIH-3T3 cells,[5] HEK293, [6] HeLa, and [7] SH-SY5Y cells. Strong band at ~35kDa corresponds to Fibrillarin protein seen in all cell line lysates. A much weaker band is seen in tissue lysates since Fibrillarin is more heavily expressed in rapidly dividing cells. The blot simultaneously was probed with rabbit pAb to UCHL1 at a dilution of 1:3,000, in red, revealing the ~25kDa UCHL1 protein in lysates of tissues containing neurons or cells with neuronal properties.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Western-Blot-NBP3-05533-img0002.jpg "Western Blot: Fibrillarin Antibody [NBP3-05533] - Western blot analysis of different tissue and cell line lysates using Fibrillarin Antibody at a dilution of 1:2,000, in green: [1] protein standard (red), [2] rat whole brain, [3] mouse whole brain, [4] NIH-3T3 cells,[5] HEK293, [6] HeLa, and [7] SH-SY5Y cells. Strong band at ~35kDa corresponds to Fibrillarin protein seen in all cell line lysates. A much weaker band is seen in tissue lysates since Fibrillarin is more heavily expressed in rapidly dividing cells. The blot simultaneously was probed with rabbit pAb to UCHL1 at a dilution of 1:3,000, in red, revealing the ~25kDa UCHL1 protein in lysates of tissues containing neurons or cells with neuronal properties.")
![Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody [NBP3-05533] - Immunofluorescent analysis of (A) C6 rat glioma cells and (B) HEK293 human embryonic kidney cells stained with Fibrillarin Antibody at a dilution of 1:1,000 in red, in both cases costained with chicken pAb to vimentin at a dilution of 1:10,000 in green. The blue is DAPI staining of nuclear DNA. The Fibrillarin antibody detects Fibrillarin protein localized in nucleoli while the vimentin antibody produces strong staining of cytoplasmic intermediate filaments.](http://images.novusbio.com/fullsize/Fibrillarin-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05533-img0001.jpg)